Figure 2. Cell surface down regulation of CXCR4 in XHR transduced Magi-CXCR4 cells. Magi-CXCR4 cells that constitutively express CXCR4 were transduced with control GFP or XHR vectors. Cells were stained with PECy5-conjugated antibodies to CXCR4 and analyzed by FACS 72 hours post-transduction. Levels of CXCR4 in non-transduced cells are superimposed (unshaded areas). Transduction efficiency was determined by FACS for EGFP expression. Levels of EGFP in control GFP-alone vector (A) and XHR vector (C) transduced cells. Levels of CXCR4 expression in GFP-alone (B) and XHR (D) vector transduced cells. Percent positive cells are indicated.