Figure 3. Imaging peripheral arbor re-innervation in vivo. Confocal projections showing dorsal views of two trigeminal axons, visualized with GFP, in a 54 hpf zebrafish embryo at several time points during a two-photon axotomy experiment. Anterior is to the bottom left. Images are each 420 microns across. (a) Twenty minutes before axotomy. (b) Approximately 20 minutes after axotomy. Yellow arrow points to site of axotomy. (c) Two hours after axotomy, the distal portion degenerates. (d) Robust regrowth is apparent 12 hours after axotomy. Figure provided by A Sagasti.