Chemokine receptors in the rheumatoid synovium: upregulation of CXCR5
Schmutz, C., Hulme, A., Burman, A., Salmon, M., Ashton, B., Buckley, C., Middleton, J. (2004) Arthritis Research & Therapy.
ABSTRACT
In patients with rheumatoid arthritis (RA), chemokine and chemokine receptor interactions play a central role in the recruitment of leukocytes into inflamed joints. This study was undertaken to characterize the expression of chemokine receptors in the synovial tissue of RA and non-RA patients. RA synovia (n = 8) were obtained from knee joint replacement operations and control non-RA synovia (n = 9) were obtained from arthroscopic knee biopsies sampled from patients with recent meniscal or articular cartilage damage or degeneration. The mRNA expression of chemokine receptors and their ligands was determined using gene microarrays and PCR. The protein expression of these... Show Full AbstractFigures From Article (6)
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Figure 1. Microarray analysis of chemokine and chemokine receptor expression in the rheumatoid arthritis (RA) and non-RA synovia. A pair of human cytokine array membranes were hybridized to 33P-labelled cDNA probes prepared from pools of (a) RA mRNA (n = 8) and... Show Full Caption 
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Figure 2. RT-PCR on rheumatoid arthritis and non-rheumatoid arthritis synovial tissue. CXCR5 RT-PCR products were separated on 0.8% agarose gels and stained with ethidium bromide. The reactions were performed on the individual RNA samples that were applied to the microarray membranes. The... Show Full Caption 
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Figure 3. Immunohistochemistry of CXCR5 in lymphoid cell aggregates of rheumatoid arthritis synovia. Sections of rheumatoid synovium were treated with (a) CXCR5 antibody or (b) isotype control. Serial sections were treated with (c) anti-CD20 as a marker of B lymphocytes or (d)... Show Full Caption 
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Figure 4. Immunohistochemistry of CXCR5 in the intima and postcapillary venules in rheumatoid arthritis synovia. (a) CD68+ cells in the intima. (b) Serial section to (a) stained for CXCR5. Note the colocalization of CXCR5 and CD68 to the same group of cells.... Show Full Caption 
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Figure 5. Immunohistochemistry of CXCR5 in non-rheumatoid arthritis synovia. (a) CD68 staining in the intimal layer. (b) Serial section to (a) treated with anti-CXCR5, showing that CXCR5+ cells in the intimal layer included those also positive for CD68. (c) and (d) Sections... Show Full Caption 
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Figure 6. RT-PCR on monocytes/macrophages from peripheral blood (PB) and synovial fluid (SF). CXCR5 RT-PCR products were separated on 0.8% agarose gels and stained with ethidium bromide. The reactions were performed on four additional rheumatoid arthritis patients. The ribosomal RNA L27 was employed to normalize the amount of RNA used in each reaction. Tables From Article (1)
TABLE CAPTION
Table 1. Details of rheumatoid arthritis (RA) and non-RA patients | Patient (sex, age [years]) | Diagnosis/pathology | Disease duration (years) | Medication |
| 1 (male, 69) | RA | 37 | Auranofin, NSAID |
| 2 (female, 41) | RA | 23 | NSAID |
| 3 (female, 51) | RA | 10 | NSAID, analgesic |
| 4 (female, 79) | RA | 4 | Methotrexate, NSAID, steroid |
| 5 (female, 70) | RA | 8 | Penicillamine, steroid, NSAID |
| 6 (female, 63) | RA + secondary osteoarthritis | 39 | Methotrexate, steroid, analgesic |
| 7 (female, 33) | juvenile chronic arthritis | 20 | NSAID |
