Differential susceptibility of naïve, central memory and effector memory T cells to dendritic cell-mediated HIV-1 transmission
Groot, F., van Capel, T., Schuitemaker, J., Berkhout, B., de Jong, E. (2006) Retrovirology.
ABSTRACT
Figures From Article (5)
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Figure 1. T cell subsets differ in susceptibility to DC-mediated transmission of R5 and X4 HIV-1. (A) DC were incubated with R5 or X4 HIV-1, or mock treated, followed by extensive washing to remove unbound virus. DC were subsequently co-cultured with CD4+... Show Full Caption 
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Figure 2. DC-mediated HIV-1 transmission is co-receptor dependent. (A) FACS analysis of TN, TCM and TEM cells for CD4 and co-receptors CCR5 and CXCR4. Open histograms represent isotype controls. (B) Transmission inhibition by co-receptor ligands and a fusion inhibitor. A single-cycle transmission... Show Full Caption 
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Figure 3. Spreading infection assay. Replication of R5 (A) and X4 (B) virus in TN, TCM and TEM cells after DC-mediated HIV-1 transmission. Alternatively, the T cell subsets were stimulated by crosslinking CD3/CD28 with antibodies and infected with R5 (C) or X4... Show Full Caption 
FIGURE CAPTION
Figure 4. Method of T cell stimulation determines HIV-1 susceptibility. (A) Comparison of viral replication in TN, TCM and TEM cells that were stimulated by DC or by CD3/CD28 crosslinking with antibodies. The T cells were stimulated for 4 days, harvested and... Show Full Caption 
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Figure 5. DC transmit HIV-1 with equal efficiency to Th0, Th1 and Th2 cells. (A) In vitro generated polarized populations of Th1 and Th2 cells, or an unbiased population (Th0), were analyzed for intracellular cytokines IFNγ and IL-4 by FACS. The percentage... Show Full Caption Tables From Article (0)
