EGFR Expression in Gallbladder Carcinoma in North America
Kaufman, M., Mehrotra, B., Limaye, S., White, S., Fuchs, A., Lebowicz, Y., Nissel-Horowitz, S., Thomas, A. (2008) International Journal of Medical Sciences.
FIGURE CAPTION
Figure 1. EGFR Staining.
Expression of the epidermal growth factor receptor (EGFR) and the phosphorylated EGFR in invasive breast carcinomas
Magkou, C., Nakopoulou, L., Zoubouli, C., Karali, K., Theohari, I., Bakarakos, P., Giannopoulou, I. (2008) Breast Cancer Research : BCR.
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Figure 1. Immunohistochemisty of EGFR and pEGFR in invasive breast carcinoma. Intense membrane staining of (a) EGFR (ABC/HRPX200) and of (b) pEGFR (ABC/HRPX400) in two cases of invasive ductal breast carcinoma. EGFR, epidermal growth factor receptor; pEGFR, phosphorylated epidermal growth factor receptor.
Expression of the epidermal growth factor receptor (EGFR) and the phosphorylated EGFR in invasive breast carcinomas
Magkou, C., Nakopoulou, L., Zoubouli, C., Karali, K., Theohari, I., Bakarakos, P., Giannopoulou, I. (2008) Breast Cancer Research : BCR.
FIGURE CAPTION
Figure 2. Univariate analysis (log-rank test) of overall survival. Schematic representation of the impact of (a) EGFR, (b) pEGFR and (c) EGFR/pEGFR expression on overall survival (OS). EGFR, epidermal growth factor receptor; pEGFR, phosphorylated epidermal growth factor receptor.
Is EGFR expression altered following postoperative chemotherapy for colorectal adenocarcinoma
Khalifa, M., Rowsell, C., Gladdy, R., Ko, Y., Hanna, S., Smith, A., Law, C. (2006) World Journal of Surgical Oncology.
FIGURE CAPTION
Figure 1. EGFR immunopositivity in > 50% of cells in a metastatic colorectal adenocarcinoma to the liver (original magnification × 200).
Functional analysis of cancer-associated EGFR mutants using a cellular assay with YFP-tagged EGFR intracellular domain
de Gunst, M., Gallegos-Ruiz, M., Giaccone, G., Rodriguez, J. (2007) Molecular Cancer.
FIGURE CAPTION
Figure 3. Evaluating erlotinib sensitivity of EGFR mutants in the context of YFP-EGFR-ICD. A. Representative examples of MCF-7 cells expressing YFP-EGFR-ICD wild type, Del746 or Del746/T790M (green), stained for endogenous phosphorylated Akt (red). Cells were treated for 20 hours with the indicated... Show Full Caption
Functional analysis of cancer-associated EGFR mutants using a cellular assay with YFP-tagged EGFR intracellular domain
de Gunst, M., Gallegos-Ruiz, M., Giaccone, G., Rodriguez, J. (2007) Molecular Cancer.
FIGURE CAPTION
Figure 1. Common NSCLC-associated EGFR mutations lead to increased autophosphorylation of an YFP-tagged EGFR intracellular domain. A. Schematic representation of full-length EGFR and YFP-EGFR-ICD. The amino acid numbering includes the 24 residues of the signal peptide. YFP-EGFR-ICD contains the tyrosine... Show Full Caption
Induction of BIM Is Essential for Apoptosis Triggered by EGFR Kinase Inhibitors in Mutant EGFR-Dependent Lung Adenocarcinomas
Gong, Y., Somwar, R., Politi, K., Balak, M., Chmielecki, J., Jiang, X., Pao, W. (2007) PLoS Medicine.
FIGURE CAPTION
Figure 7. In Vivo Correlation of BIM/Bim Expression and Mutant EGFR Activity (A and B) Lung tumor-bearing mice that express a tetracycline-inducible L858R mutant (EGFRL858R) (A) or an exon 19 deletion mutant... Show Full Caption
EGFR Inhibitor Enhances Cisplatin Sensitivity of Oral Squamous Cell Carcinoma Cell Lines
Hiraishi, Y., Wada, T., Nakatani, K., Tojyo, I., Matsumoto, T., Kiga, N., Negoro, K., Fujita, S. (2008) Pathology Oncology Research .
FIGURE CAPTION
Fig. 3. Total expression of epidermal growth factor receptor (EGFR) and phosphorylated receptor (p-EGFR). Total expression of EGFR and p-EGFR was determined by Western blot analysis. Ten micrograms of total cellular lysates prepared from parental and cisplatin (CDDP)-resistant cells were resolved by electrophoresis, transferred to a PVDF membrane, and blotted with anti-EGFR and anti-p-EGFR antibodies as described in the Materials and methods section
Jab1 is a target of EGFR signaling in ERα-negative breast cancer
Wang, J., Barnes, R., West, N., Olson, M., Chu, J., Watson, P. (2008) Breast Cancer Research : BCR.
FIGURE CAPTION
Figure 5. Jab1 nuclear expression correlates with increased EGFR and S100A7 in breast tumors. EGFR, S100A7, and Jab1 were detected by immunohistochemistry in breast tumors represented within a tissue microarray. Representative staining is shown for all three markers and in three separate tumors.... Show Full Caption
The natural history of EGFR and EGFRvIII in glioblastoma patients
Heimberger, A., Suki, D., Yang, D., Shi, W., Aldape, K. (2005) Journal of Translational Medicine.
FIGURE CAPTION
Figure 1. Graph showing Kaplan-Meier estimates of overall survival in glioblastoma multiforme patients who underwent sub-total resection followed by standard-of-care radiation therapy and chemotherapy. Patients with tumors not expressing the epidermal growth factor receptor (EGFR; n = 23; solid black line), expressing amplified EGFR (n = 14; dashed grey line), and expressing EGFRvIII (n =... Show Full Caption
Functional analysis of cancer-associated EGFR mutants using a cellular assay with YFP-tagged EGFR intracellular domain
de Gunst, M., Gallegos-Ruiz, M., Giaccone, G., Rodriguez, J. (2007) Molecular Cancer.
FIGURE CAPTION
Figure 2. Activation of Akt and Erk pathways in cells expressing mutant YFP-EGFR-ICD Del746. A. Panels show representative images (400X) of MCF-7 cells expressing YFP-EGFR-ICD wild type, Del746 or V948R, analyzed by immunofluorescence to detect phosphorylated Akt (pAkt). Phosphorylation of endogenous Akt was... Show Full Caption
Id-1: Regulator of EGFR and VEGF and potential target for colorectal cancer therapy
Meteoglu, I., Meydan, N., Erkus, M. (2008) Journal of Experimental & Clinical Cancer Research : CR.
FIGURE CAPTION
Figure 1. Differential staining intensity of Id-1, EGFR and VEGF between non-tumoural colorectal tissues and colorectal carcinoma specimens (A-E-I). 2-Images of Id-1 (B-C-D), EGFR (F-G-H) and VEGF (J-K-L) expressions in different grades of colorectal carcinoma. Note that increased Id-1, EGFR and VEGF expressions are associated with increased tumour grade.
Functional analysis of cancer-associated EGFR mutants using a cellular assay with YFP-tagged EGFR intracellular domain
de Gunst, M., Gallegos-Ruiz, M., Giaccone, G., Rodriguez, J. (2007) Molecular Cancer.
FIGURE CAPTION
Figure 4. Testing the kinase activity and erlotinib sensitivity of uncommon EGFR mutants using the YFP-EGFR-ICD assay. A. Partial amino acid sequence of EGFR exon 20 and exon 21 illustrating the location of the mutations examined (red letters). B. Kinase activity and... Show Full Caption
Radiation-induced caveolin-1 associated EGFR internalization is linked with nuclear EGFR transport and activation of DNA-PK
Dittmann, K., Mayer, C., Kehlbach, R., Rodemann, H. (2008) Molecular Cancer.
FIGURE CAPTION
Figure 1. Radiation induced caveolin-1 linked EGFR internalization is triggered by src kinase. Confluent A549 cells were irradiated with 4 Gy (A). At the time points given, src protein was immune-precipitated from cytoplasmic protein fraction. Proteins were separated by SDS-PAGE and after blotting the... Show Full Caption
Radiation-induced caveolin-1 associated EGFR internalization is linked with nuclear EGFR transport and activation of DNA-PK
Dittmann, K., Mayer, C., Kehlbach, R., Rodemann, H. (2008) Molecular Cancer.
FIGURE CAPTION
Figure 2. Stabilization of caveolin1/EGFR complex in cytoplasm after incubation with EGFR inhibitory antibody Erbitux. Confluent A549 cells were treated with human IgG or EGFR inhibitory antibody Erbitux at a concentration of 30 nM for 1 h. Subsequently cells were irradiated with 4... Show Full Caption
Induction of BIM Is Essential for Apoptosis Triggered by EGFR Kinase Inhibitors in Mutant EGFR-Dependent Lung Adenocarcinomas
Gong, Y., Somwar, R., Politi, K., Balak, M., Chmielecki, J., Jiang, X., Pao, W. (2007) PLoS Medicine.
FIGURE CAPTION
Figure 1. Effects of Erlotinib on EGFR Signaling Pathways in H3255, PC-9, H1650, and H1975 Cells Cells were treated with the indicated concentrations of erlotinib for 6 h. Cell lysates were analyzed by immunoblotting, using the indicated antibodies. t, total protein; p, phospho-protein.
Stratification of non-small cell lung cancer patients for therapy with epidermal growth factor receptor inhibitors: the EGFR fluorescence in situ hybridization assay
Varella-Garcia, M. (2006) Diagnostic Pathology.
FIGURE CAPTION
Figure 2. Distinct types of EGFR gene amplification identified in non-small cell lung carcinomas using the EGFR SpectrumOrange/CEP 7 SpectrumGreen FISH probe (Vysis/Abbott Molecular): A. Large EGFR gene clusters; B. Co-localized clusters of EGFR and CEP7 signals; C. Large and bright EGFR signal, larger than the CEP 7 signals in tumor cells; D. High frequency of balanced EGFR and CEP 7 signals.
Genomic activation of the EGFR and HER2-neu genes in a significant proportion of invasive epithelial ovarian cancers
Vermeij, J., Teugels, E., Bourgain, C., Xiangming, J., in 't Veld, P., Ghislain, V., Neyns, B., De Grève, J. (2008) BMC Cancer.
FIGURE CAPTION
Figure 1. Immunohistochemical and FISH analysis of EGFR and HER2-neu on ovarian tumours. A-C : Immunohistochemistry for EGFR and HER2-neu protein expression on paraffin sections from ovarian tumours (all 400× magnification). Examples of an EGFR score 0 (A), EGFR 3+ overexpression... Show Full Caption
EGFR associated expression profiles vary with breast tumor subtype
Hoadley, K., Weigman, V., Fan, C., Sawyer, L., He, X., Troester, M., Sartor, C., Rieger-House, T., Bernard, P., Carey, L., Perou, C. (2007) BMC Genomics.
FIGURE CAPTION
Figure 5. EGFR pathway diagram displayed for each breast tumor subtype. The average gene expression value for each gene within each subtype is displayed for the EGFR-pathway and for the three EGFR-activation profiles using the UNC 248 tumor dataset. Eight genes from the middle... Show Full Caption
Induction of BIM Is Essential for Apoptosis Triggered by EGFR Kinase Inhibitors in Mutant EGFR-Dependent Lung Adenocarcinomas
Gong, Y., Somwar, R., Politi, K., Balak, M., Chmielecki, J., Jiang, X., Pao, W. (2007) PLoS Medicine.
FIGURE CAPTION
Figure 4. BIM Status Is Dramatically Altered by Inhibition of EGFR Kinase Activity in Cells Dependent upon Mutant EGFR Signaling for Survival (A) Effects of erlotinib treatment on proapoptotic and antiapoptotic proteins... Show Full Caption